Welcome to the Plant Single Cell Laboratory at the University of Nebraska–Lincoln directed by Associate Professor Marc Libault. Working on single plant cells, the laboratory investigates the genomic and epigenomic regulation of gene expression in response to various abiotic and biotic stresses.

Specifically, applying modern functional genomic and nucleic acid sequencing tools, we are characterizing the interactions between transcription factors and the genomic DNA, the regulation and function of small RNAs on gene transcription. The various projects developed in the laboratory can be accessed by clicking on the “Projects” and “Publications".

Plant single nuclei RNA-seq (sNucRNA-seq) library construction using 10x Genomics technology

Courtesy of Emily Tran and Dr. Colleen Syron, University of Nebraska-Lincoln

  • Ms./Ph.D. Position in Plant Single Cell Computational Biology at the University of Nebraska-Lincoln is now open

    The graduate student will apply and develop bioinformatics and computational tools to reveal the transcriptomic regulation of plant genes in response to biotic and abiotic stresses at the level of single plant cells under the co-advisory of Drs. Libault and Saha (https://engineering.unl.edu/ssbio/).

    Visit Job openings for more information and to apply

  • OFFERED SPRING 2022 AGRO 429/829
    Plant Biotechnology Applications Instructor: Dr. Libault

    For class schedule and syllabus got to Teaching

  • Ian and Akash, Lincoln High School Students, share an outstanding video of their summer research project.

    Ian and Akash decided to continue their research project in the lab during the Fall semester.

    Open access

  • Congratulation to Luke Gohmann (2021 REU student from Idiana University) for winning best poster presentation!

  • In the news: 
    Marc Libault
    Husker scientist leads effort to understand, adapt legume nitrogen conversion

    Soybeans and other legumes interact with nitrogen-fixing soil bacteria called rhizobia that are able to convert nitrogen in the air into a form the plant can use to grow and reproduce. Corn and other crops can’t, requiring nitrogen fertilizers to maximize growth and yield — problematic because overapplication or runoff can pollute soil and water.

  • New publication from the Libault lab in Molecular Plant

    Open access
    DOI:https://doi.org/10.1016/j.molp.2021.01.001

  • 2019 ARD Innovation Fund for Wheat/Cereal Crops Award

    The long-term goal of this project is to elucidate the impact of gene expression on wheat heterosis for yield by revealing the differential use of the genomic information between parents and the F1 progeny. To maximize the level of resolution of our transcriptomic analysis, we are applying single cell RNA-seq technology on wheat leaves. The work will be done is collaboration with Pr. Baenziger and Dr. Belamkar (Department of Agronomy and Horticulture, UNL).
  • Thank you Emily (REU program) for your hard work in the lab

    Emily Behling, Undergraduate student at St. Olaf College in Northfield Minnesota, has been selected as a participant in the Plant and Microbiome Summer Research Program. This program is funded by the National Science Foundation (NSF) and Nebraska EPSCoR. Emily is working on the integration and comparison of transcriptomic, metabolomics and phenotypic datasets between various maize cultivars
  • Single Plant Cell Lab

    The laboratory is using single plant cell type models to investigate plant response to biotic and abiotic stresses