Welcome to the Plant Single Cell Laboratory at the University of Nebraska–Lincoln directed by Associate Professor Marc Libault. Working on single plant cells, the laboratory investigates the genomic and epigenomic regulation of gene expression in response to various abiotic and biotic stresses.

Specifically, applying modern functional genomic and nucleic acid sequencing tools, we are characterizing the interactions between transcription factors and the genomic DNA, the regulation and function of small RNAs on gene transcription. The various projects developed in the laboratory can be accessed by clicking on the “Projects” and “Publications".

Plant single nuclei RNA-seq (sNucRNA-seq) library construction using 10x Genomics technology

Courtesy of Emily Tran and Dr. Colleen Syron, University of Nebraska-Lincoln

  • New publication from the Libault lab in Molecular Plant

    Single-nucleus RNA and ATAC sequencing reveals the impact of chromatin accessibility on gene expression in Arabidopsis roots at the single-cell level

    Open access

  • Welcome Michael Israel in the lab!

    Michael is a graduate student in the Complex Biosystems Program at UNL.
  • 21st International Congress On Nitrogen Fixation in Wuhan, China

    Presentation of our most recent discoveries using single nuclei -omics technologies on plant roots.

    Dr. Libault met with a fellow collaborator Dr. Yan Zhe during the Congress.
  • 2019 ARD Innovation Fund for Wheat/Cereal Crops Award

    The long-term goal of this project is to elucidate the impact of gene expression on wheat heterosis for yield by revealing the differential use of the genomic information between parents and the F1 progeny. To maximize the level of resolution of our transcriptomic analysis, we are applying single cell RNA-seq technology on wheat leaves. The work will be done is collaboration with Pr. Baenziger and Dr. Belamkar (Department of Agronomy and Horticulture, UNL).
  • Thank you Emily (REU program) for your hard work in the lab

    Emily Behling, Undergraduate student at St. Olaf College in Northfield Minnesota, has been selected as a participant in the Plant and Microbiome Summer Research Program. This program is funded by the National Science Foundation (NSF) and Nebraska EPSCoR. Emily is working on the integration and comparison of transcriptomic, metabolomics and phenotypic datasets between various maize cultivars
  • In the news: 
    Marc Libault
    Nebraska plant scientist digs deeper into soybeans and soil bacteria

    Nebraska's Marc Libault, who received a Faculty Early Career Development Program award from the National Science Foundation, seeks to better understand soybeans and other legumes’ mutually beneficial relationship with the bacteria rhizobia. His research may lead to a reduction in the need for costly fertilizers.

  • Single Plant Cell Lab

    The laboratory is using single plant cell type models to investigate plant response to biotic and abiotic stresses