The ultrasound aeroponic system feeds the plants with a fine cool mist of nutritive solution promoting root hair cell emergence. This system allow an easy access to root hair cells for both microscopic observations and their isolation before applying functional genomic tools.
Using liquid nitrogen, as much as 1 gram of root hair cells can be isolated from 60 plants growing in the ultrasound aeroponic fogger system. This unique plant material allows us to investigate the response of one single plant cell type to various abiotic and biotic stresses.
The lab developed a protocol to isolate nuclei fraction compatible with single-nucleus RNA-sequencing library construction using microfluidic system.