Protocols

Ultrasound Aeroponic System

mist

The ultrasound aeroponic system feeds the plants with a fine cool mist of nutritive solution promoting root hair cell emergence. This system allow an easy access to root hair cells for both microscopic observations and their isolation before applying functional genomic tools.

Root Hair Cell Isolation

root hair isolation

Using liquid nitrogen, as much as 1 gram of root hair cells can be isolated from 60 plants growing in the ultrasound aeroponic fogger system. This unique plant material allows us to investigate the response of one single plant cell type to various abiotic and biotic stresses.

Molecular Biology

In the laboratory, we are using various molecular tools to investigate the response of the root hair cells to stresses and to characterize the function of selected soybean genes in controlling those responses.