BIOL/MBIO/PBIO 4843/5843: Molecular Biology


MBIO-PBIO 4263: Cell Biology Laboratory

Course Description

Students will develop improved writing and thinking skills with a working knowledge of cell biology. Particularly we seek to accomplish the following for course enrollees (i.e. future researchers and physicians):

  • Reinforce the skills and procedures of keeping a lab notebook and formal lab reporting.
  • Develop critical thinking skills and the ability to apply these to planning experiments and writing about cell biology.
  • Solidify the basic common skills used in cell biology and laboratory techniques (lab calculations, sterile technique, bacteria production, setting up reactions, quantifying nucleic acids, handling quantitative data, etc.).

During this semester, you will work on molecular and cellular techniques to analyze:

    • Cloning of DNA sequences in appropriate vectors
    • Quantification and validation of the quality of nucleic acid samples
    • Immunodetection of proteins
    • Gene transcriptional activity at the level of a tissue and single cell
    • Protein sub-cellular localization
    • Microscopic observation
    • Bioinformatics

Genomics class

PBIO-MBIO 4313: Biotechnology Applications

Course Description

Biotechnology is the multidisciplinary approach of applying organisms and biological molecules to specific uses. At an ever-increasing pace public and private sector scientists have applied biotechnology to help meet social needs. Intended for students who possess a working knowledge of molecular biology, this course will focus of developing familiarity with methods used in biotechnology to address pressing societal challenges. Participants will put into practice central methods of biotechnology, gaining practical skills for use in future careers in laboratory science. In particular, we will cover methods relevant to pharmaceutical production, agricultural improvement, biofuel production, and medical and forensic diagnostics, among others.

Biotech class

PBIO-MBIO 4810: Introduction to Genomics: from the gene to the organism

Course Description

What are the differences between your DNA sequences and the DNA sequences from other animals?

What are the molecular mechanisms that control the expression of genes?

How do researchers investigate gene function?

How do computational tools impact genomic studies?


The purpose of this class is to introduce the organization and evolution of genomes through the following topics:

      1. Genes and the organization of the nucleus
      2. Sequencing of genomes
      3. Computational and comparative genomics; Organization and evolution of genomes; SNPs
      4. Functional genomics: characterization of the function of the genes; molecular studies of gene expression
      5. Introduction to the concept of “epigenome”

This 3000-level course is intended for plant biology, microbiology, biology, and biochemistry students interested in the study of the entire genome of prokaryotic and eukaryotic organisms. Through discussions,, reading of literature as well as applied exercises, you will study the organization and evolution of genomes and will discover how researchers currently study gene function.


REHSS Program

Course Description

the “Research Experiences for High School Students” (REHSS), a plant biotechnology educational program created in 2012 and dedicated to introducing Oklahoma high-school students to modern biology. To date, the REHSS program allowed over 100 high-school students to participate in workshops organized in three different Oklahoma high schools, and 30 students were hosted at OU laboratories for an 8-weeks research experience.